Interfacing MALDI

SimulTOF Systems is engaged is multiple initiatives aimed at developing products that will enable transition from peptide and protein separations to MALDI TOF (TOF/TOF) mass spectrometry.

Liquid Chromatography

LC-MALDI workflows for proteomics utilizing “top-down” and “bottom-up” methodologies are being carried out with on-going in-house and collaborative investigations.  

The decoupling of separation from mass analysis offered by LC-MALDI protocols enables the chromatography and the mass spectrometer to be optimized independently for maximum efficiency. Free from the time constraints inherent in “on-line” LCMS techniques, LC-MALDI protocols allow for LC runs to be scanned in MS mode and the resulting parent-mass information can then be used in a true data dependent manner to set up the MS-MS measurements. Since it is rare for all sample to be used in most MALDI measurements, additional measurements can be made at any later time as needed.

Links to Related Presentations

  1. Comparison of chicken light and dark meat - ASMS 2011
  2. Peptide Mass Fingerprinting - ASMS 2011

Monolithic column technology was utilized in the construction of 3-dimensional MALDI plates for interfacing high flow, high capacity LC separations with MALDI.   The 3-D plate design effectively captures analyte of interest on a porous polymer surface while allowing carrier solvents to pass through.

Post capture analytes are eluted to the plate surface and analyzed directly in the mass spectrometer. The 3D plate design has demonstrated effective capture of analyte resulting in significant increase in MS signal intensity with full sample recovery.

Links to Related Presentations

  1. New 3D MALDI Plates - ASMS 2009
  2. 3 Dimensional MALDI Plates - ASMS 2009
  3. Novel 3D Sample Plate - ASMS 2008

 

Gel electrophoresis

The coupling of gel electrophoresis to MALDI also utilizes porous hydrophobic polymers to capture gel separated proteins. MALDI plates designed for this application pay particular attention to the maintenance of spatial resolution.

Glass µchannel plates (25µm) as well as plates constructed around reticulated vitreous carbon foam have been utilized for interfacing electrophoretic separations with MALDI.

Links to Related Presentations

  1. Carbon Plate - ASMS 2011
  2. High Resolution MALDI plates - ASMS 2010