The SimulTOF TWO is a linear bipolar MALDI-TOF mass spectrometer which provides high-speed molecular imaging of both small and large molecules in both positive and negative ion modes.

  • Performance for proteins and oligonucleotides equivalent to SimulTOF ONE

  • Longer flight tube and programable voltages provide higher resolution for peptides and metabolites

  • Very high sensitivity, precision, and dynamic range for both positive and negative ions

  • Provides isotopic resolution below ca. 6 kDa, and isotopic envelope resolution for high masses


Image of full porcine pancreas at 200 pixels/second showing Islets of Langerhans. Spatial Resolution is 10 mm with single laser shot/pixel.

Islets of Langerhans are irregularly shaped patches of endocrine tissue located within the pancreas of most vertebrates. They are named for the German physician Paul Langerhans, who first described them in 1869.

The islets of Langerhans contain alpha, beta, and delta cells that produce glucagon, insulin, and somatostatin, respectively. A fourth type of islet cell, the F (or PP) cell, is located at the periphery of the islets and secretes pancreatic polypeptide.

Langerhans on display of the website
Simutof two on display of the website


Ion Energy: +20 kV, -10kV
Vacuum System
  • Dual turbomolecular vacuum pumps

  • 24 VDC Mechanical Pump

  • Patented fast plate loading <30 seconds load to operate

  • Quick Clean Ion Accelerator—1 minute hands on, 1 hour pump down

  • 23.6″ / 600 mm width

  • 27.5″ / 700 mm depth

  • 64.7″ / 1643 mm height

  • 340 lb / 154 kg weight

  • Solid-state Nd:YLF @349 nm

  • 0.1-5 kHz standard with computer controlled fluence 1-30 μJ

  • Number of shots before maintenance >1012

  • Laser spot dia 5-30 μm

Imaging Speed
  • Up to 300 pixels/sec

  • Resolution and sensitivity over 3-100 kDa mass range, superior to all other linear bi-polat MALDI-TOF instruments