SimulTOF ONE

SimulTOFOne

The SimulTOF ONE Linear MALDI-TOF mass spectrometer is ideally suited for intact proteins, peptides, oligonucleotide, other biomolecules, and for quality control of small molecule synthesis. The instrument is fully automated and is designed for ease-of-use through intuitive software that requires only minimal training for first-time users of MS.

New concepts in instrument design have produced a system that is simple, reliable, and robust with only minimal preventative maintenance required. This a true bench top design, including a turbo molecular pump and a self-contained roughing pump and both the mass spectrometer and the computer are powered from a single 20A circuit. No other utilities are required.

  • Highest performance available from any linear MALDI-TOF MS over full mass range to 1,000,000 Da.
  • Smallest footprint of any linear MALDI-TOF
  • High performance hybrid detector provides excellent mass range, speed and dynamic range.
  • Standard Options include 5 kHz laser with 2 GHz digitizer; bipolar ion analyzer; and very fast plate autoloader for high throughput applications.
  • Highest speed molecular Imaging with high spatial resolution
  • Technology developed and patented at SimulTOF produces robust MALDI-TOF mass spectrometers with no compromise in performance at an affordable price.

Spot Chromatogram
Image of 2.6 mm spot (450,000 laser shots) with 25 µm pixels, HCCA matrix, 25 mm raster @ 2 mm/s, 7700 pixels @ 56 shots/pixel saved, 5 kHz laser, 90 pixels/s, 90 s acquisition

Standard Applications Software includes:

  • Hemoglobin high throughput A1C assay with 1% CV
  • Assay of albumin in urine with high precision and dynamic range
  • Quantitative analysis of complex mixtures
  • Mass spectroscopic immunoassay (MSIA)
  • Interface of gel separations with high performance MS on intact proteins
  • Interface with liquid handling robots using microtiter formats
  • Protein identification by matching DNA databases
  • Quantitative analysis of complex mixtures separated by liquid chromatography
  • Up to 100 spectra/second recorded and processed

E coli spectrum
Superposition of spectra from four spots of E coli in HCCA matrix                                          Expanded View